Chemical Communications p. 4786 - 4789 (2018)
Update date:2022-08-16
Topics:
Sedgwick, Adam C.
Gardiner, Jordan E.
Kim, Gyoungmi
Yevglevskis, Maksims
Lloyd, Matthew D.
Jenkins, A. Toby A.
Bull, Steven D.
Yoon, Juyoung
James, Tony D.
Two 'turn on' TCF-based fluorescence probes were developed for the detection of biological thiols (TCF-GSH and TCFCl-GSH). TCF-GSH was shown to have a high sensitivity towards glutathione (GSH) with a 0.28 μM limit of detection. Unfortunately, at higher GSH concentrations the fluorescence intensity of TCF-GSH decreased and toxicity was observed for TCF-GSH in live cells. However, TCFCl-GSH was shown to be able to detect GSH at biologically relevant concentrations with a 0.45 μM limit of detection. No toxicity was found for TCFCl-GSH and a clear 'turn on' with good photostability was observed for the exogenous addition of GSH, Cys and HCys. Furthermore, TCFCl-GSH was used to evaluate the effects of drug treatment on the levels of GSH in live cells.
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