Lipo-oligosaccharide of the Campylobacter lari type strain ATCC 35221. Structure of the liberated oligosaccharide and an associated extracellular polysaccharide

Article abstract of DOI:10.1016/0008-6215(95)00284-7

Lipo-oligosaccharide (LOS) from phenol-water extraction of cells of the Campylobacter lari type strain (ATCC 35221) was separated as a water-insoluble gel of low relative molecular mass (M_r) from a water-soluble extracellular polysaccharide of high M_r.Structural investigations were performed on the liberated oligosaccharide and the extracellular polysaccharide, variously using 1H, 13C, 31 P NMR spectroscopy, linkage analysis, and fast atom bombardment-mass spectrometry of permethylated derivatives of the glycans and their products of chemical and enzymic degradation.The following structures are proposed for the highly branched region of the LOS: and for the tetraglycosyl phosphate repeating unit of the extracellular polysaccharide: <-(PO3^- -> 3)-β-D-Glc pNAc-(1 -> 2)-6-d-α-L-gul-Hep p-(1 -> 2)-3-d-β-D-threo-Pen p-(1 -> 3)-6-d-α-L-gul-Hep p->_n - Keywords: Campylobacter lari type strain (ATCC 35221); Lipo-oligosaccharide; Extracellular polysaccharide; Tetraglycosyl phosphate polymer