SYNTHESIS OF VARIOUS AROMATIC AMIDE DERIVATIVES USING NITRILE HYDRATASE OF RHODOCOCCUS RHODOCHOROUS J1

Article abstract of DOI:10.1016/0040-4020(89)80133-4

Nitrile hydratase, that is produced abundantly in cells of Rhodococcus rhodochorous J1, catalyses the conversion of various aromatic nitrile derivatives to the corresponding amides.Using Rh. rhodochorous J1 resting cells, the conditions for the production of benzamide, 2,6-difluorobenzamide, indolacetamide, thiophenecarboxamide and furanecarboxamide were optimized.Under the determined conditions, 489 g of benzamide, 306 g of 2,6-difluorobenzamide, 1045 g of 3-indoleacetamide, 210 g of 2-thiophenecarboxamide and 522 g of 2-furanecarboxamide were produced, with 100percent molar conversion, from the corresponding nitriles, per litre of reaction mixture.