Enantioselective oxidation of racemic citronellol with an interface bioreactor

Article abstract of DOI:10.1271/bbb.60.83

Microbial oxidation of racemic citronellol was done on the interface between a nutrient agar plate and decane (interface bioreactor). While Rhodococcus accumulated (R)-citronellal (21-80% e.e.), many yeasts accumulated (S)-citronellic acid (20-68% e.e.) as the oxidation product. For the production of (R)-citronellal and (S)-citronellic acid, Rhodococcus equi IFO 3730 and Hansenula anomala IFO 0147 were the best strains, respectively. While citronellol-oxidation in R. equi IFO 3730 was (S)-selective, citronellal-oxidation was (R)-selective. Although H. anomala had also both (S)-selective alcohol and aldehyde dehydrogenases (constitutive, NAD⁺-dependent), the equilibrium of the alcohol dehydrogenase shifted to reduction of citronellal. Geotrichum candidum JCM 01747 accumulated very high concentrations of citronellic acid (conversion yield 90% in 5 days or accumulation 65 mg/ml in 13 days) in spite of the strong toxicity of the acid, although the optical purity was low. For the alleviation of the toxicity of citronellol and accumulation of toxic citronellic acid, the interface bioreactor is superior to the organic-aqueous two-liquid-phase system (dispersion system).

Full text of DOI:10.1271/bbb.60.83

This article was downloaded by: [University of Illinois Chicago]
On: 08 December 2014, At: 16:08
Publisher: Taylor & Francis
Informa Ltd Registered in England and Wales Registered Number: 1072954 Registered office: Mortimer
House, 37-41 Mortimer Street, London W1T 3JH, UK
Bioscience, Biotechnology, and Biochemistry
Publication details, including instructions for authors and subscription information:
http://www.tandfonline.com/loi/tbbb20
Enantioselective Oxidation of Racemic Citronellol
with an Interface Bioreactor
Shinobu Odaa, Akio Katoab, Naotoshi Matsudomiab & Hiromichi Ohtaac
a Technical Research Laboratory, Kansai Paint Co., Ltd., 4-17-1 Higashi–Yawata,
Hiratsuka, Kanagawa 254, Japan
b Department of Biological Chemistry, Faculty of Agriculture, Yamaguchi University,
1677-1 Yoshida, Yamaguchi, Yamaguchi 753, Japan
c Department of Chemistry, Faculty of Science and Technology, Keio University, 3-14-1
Hiyoshi, Kohoku-ku, Yokohama, Kanagawa 223, Japan
Published online: 12 Jun 2014.
To cite this article: Shinobu Oda, Akio Kato, Naotoshi Matsudomi & Hiromichi Ohta (1996) Enantioselective Oxidation of
Racemic Citronellol with an Interface Bioreactor, Bioscience, Biotechnology, and Biochemistry, 60:1, 83-87, DOI: 10.1271/
bbb.60.83
To link to this article: http://dx.doi.org/10.1271/bbb.60.83
PLEASE SCROLL DOWN FOR ARTICLE
Taylor & Francis makes every effort to ensure the accuracy of all the information (the “Content”) contained
in the publications on our platform. However, Taylor & Francis, our agents, and our licensors make no
representations or warranties whatsoever as to the accuracy, completeness, or suitability for any purpose of
the Content. Any opinions and views expressed in this publication are the opinions and views of the authors,
and are not the views of or endorsed by Taylor & Francis. The accuracy of the Content should not be relied
upon and should be independently verified with primary sources of information. Taylor and Francis shall
not be liable for any losses, actions, claims, proceedings, demands, costs, expenses, damages, and other
liabilities whatsoever or howsoever caused arising directly or indirectly in connection with, in relation to or
arising out of the use of the Content.
This article may be used for research, teaching, and private study purposes. Any substantial or systematic
reproduction, redistribution, reselling, loan, sub-licensing, systematic supply, or distribution in any
form to anyone is expressly forbidden. Terms & Conditions of access and use can be found at http://
www.tandfonline.com/page/terms-and-conditions

NII-Electronic Library Service

NII-Electronic Library Service

NII-Electronic Library Service

NII-Electronic Library Service

NII-Electronic Library Service