Organic & Biomolecular Chemistry
Paper
adding ice-cold ether. The precipitate was washed thoroughly currently exploring the scope of their utility for various organic
with cold ether (5 × 10 mL) to remove the scavengers which were reactions as well as for combinatorial library assays.
collected and lyophilized. The second disulfide bond formation
was carried out by the air oxidation method in which the
peptide was dissolved in a 0.1 M solution of de-aerated ammo-
−
1
Acknowledgements
niumbicarbonate solution (2.5 mg mL ). The mixture was
kept open to the atmosphere with occasional stirring and the
completion of the reaction was monitored by Ellman’s test. The
peptide was dried by lyophilization and used for HPLC and
MALDI analysis.
The authors acknowledge the CSIR, New Delhi, for providing
funding and RGCB for research facilities. The help rendered
by IISc, Bangalore, for NMR and MALDI facilities is gratefully
acknowledged.
Conclusions
References
The present work detailed the synthesis, characterization
and evaluation of the PEGylated alkylamine G
2
dendrimer
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1
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Org. Biomol. Chem., 2013, 11, 4860–4870 | 4869