Hispidospermidin, a novel phospholipase C inhibitor produced by Chaetosphaeronema hispidulum (Cda) Moesz NR 7127: II - Isolation, characterization and structural elucidation

Article abstract of DOI:10.7164/antibiotics.47.6

Hispidospermidin (1) is a novel phospholipase C inhibitor produced by Chaetosphaeronema hispidulum (Cda) Moesz NR 7127. Its structure (C₂₅H₄₇N₃O) has been elucidated as a cage compound with a trimethylspermidine side chain based on various NMR studies, including ¹H-¹H COSY, ¹³C-¹H COSY, HOHAHA, HMBC, COLOC and long range J C-H resolved 2D spectroscopy. The absolute configuration of 1 has been elucidated by modified MOSHER's method on the (R)- and (S)-MTPA amides of a derivative of 1.