RADIOIMMUNOASSAY FOR THE QUANTITATIVE DETERMINATION OF HESPERIDIN AND ANALYSIS OF ITS DISTRIBUTION IN CITRUS SINENSIS

Article abstract of DOI:10.1016/0031-9422(88)80625-3

A simple and sensitive radioimmumoassay (RIA) for the citrus flavanone hesperidin and other flavonoid 7-rutinosides is described.The assay utilizes antibodies raised against a hesperidin 4-O-carboxymethyl-oxime hapten and a tritiated radiotracer prepared by direct reduction of hesperidin with NaB<3-H>4.The detection limit of the assay is 0.2 ng/0.1 ml (0.3 pmol/0.1 ml) and the measuring range extends to 10 ng/0.1 ml (16.4 pmol/0.1 ml).This assay is specific for flavonoid rutinosides, is characterized by a low coefficient of variation, and shows good correlation with HPLC for the quantification of hesperidin in oranges.The application of this method in determining the distribution of hesperidin in leaves, fruit, seeds, and seedlings of Citrus linensis is also reported.Key Word Index - Citrus sinensis; Rutaceae; radioimmunoassay; hesperidin; flavonoidglycosides; rutinosides.