IDENTIFICATION OF 3-HYDROXYOCTYL β-D-GLUCOSIDE AND ABSOLUTE CONFIGURATION OF FREE AND BOUND OCTANE-1,3-DIOL IN APPLE FRUIT

Article abstract of DOI:10.1016/0031-9422(89)85029-0

In an extract obtained from neutralized apple juice, cv.Jonathan, by LC separation on Amberlite XAD resin using ethyl acetate elution , 3-hydroxyoctyl β-D-glucoside was identified by HRGC, HRGC-MS and HRGC-FTIR after per-O-methylation.Chiral evaluation of free and glucosidically bound octane-1,3-diol was performed by HRGC after derivatization with Mosher's reagent revealing the occurrence of optically pure diol in apple fruit.By means of comparison of optical rotation of the 1,3-diacetoxy derivative with previously published data, (R)-(+)-configuration was evaluated.Key Word Index - Malus sylvestris; Rosaceae; apple; fruit; 3-hydroxyoctyl β-D-glucoside; free and bound (R)-(+)-octane-1,3-diol.