Sequence-Specific Osmium Reagents for Polynucleotides. 2. A Method for Thymine-Cytosine Pairs

Article abstract of DOI:10.1021/ja00416a014

We have modified the dinucleoside monophosphate, deoxythymidylyldeoxycytidine (d-TpC), by replacement of the exocyclic amino group of cytosine with a series of ligands, H2N(CH2)nN(CH3)CH2CH2N(CH3)2, where n = 2, 4, and 6.The kinetics of the reactions of these modified dinucleoside monophosphates with osmium tetroxide were measured.The modified nucleotides react with osmium tetroxide 4200, 7900, and 1200 times faster than the unmodified species for n = 6, 4, and 2, respectively.The products of the reactions are macrocyclic oxoosmium (VI) esters for which 360-MHz proton NMR spectra are reported.