Improvement of desulfurization activity in Rhodococcus erythropolis KA2-5-1 by genetic engineering.

Article abstract of DOI:10.1271/bbb.65.239

Rhodococcus erythropolis KA2-5-1 can desulfurize dibenzothiophene (DBT) into 2-hydroxybiphenyl. A cryptic plasmid, pRC4, which was derived from R. rhodochrous IFO3338, was combined with an Escherichia coli vector to construct an E. coli-Rhodococcus shuttle vector. The complete nucleotide sequence of 2582-bp pRC4 was analyzed. Based on the characteristics of its putative replication genes, pRC4 was assigned to the family of pAL5000-related replicons. The desulfurization gene cluster, dszABC, and the related reductase gene, dszD, cloned from KA2-5-1, were reintroduced into KA2-5-1 and efficiently expressed. The DBT desulfurization ability of the transformant carrying two dszABC clusters and one dszD on the vector was about 4-fold higher than that of the parent strain, and the transformant also showed improved desulfurization activity for light gas oil (LGO). Sulfur components in LGO before and after the reaction were analyzed with gas chromatography-atomic emission detection.

Full text of DOI:10.1271/bbb.65.239

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Improvememt of Desulfurization Activity in
Rhodococcus erythropolis KA2-5-1 by Genetic
Engineering
Kazuaki HIRASAWAa, Yoshitaka ISHIIa, Morio KOBAYASHIa, Kenichi KOIZUMIa & Kenji
MARUHASHIa
a Bio-Refining Process Laboratory, Advanced Technology and Research Institute,
Petroleum Energy Center
Published online: 22 May 2014.
To cite this article: Kazuaki HIRASAWA, Yoshitaka ISHII, Morio KOBAYASHI, Kenichi KOIZUMI & Kenji MARUHASHI (2001)
Improvememt of Desulfurization Activity in Rhodococcus erythropolis KA2-5-1 by Genetic Engineering, Bioscience,
Biotechnology, and Biochemistry, 65:2, 239-246, DOI: 10.1271/bbb.65.239
To link to this article: http://dx.doi.org/10.1271/bbb.65.239
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