Calcium-promoted hydrolysis of N-acylureas allows mild release of peptides anchored with Dpr(Phoc) linker to hydrophilic resins

Article abstract of DOI:10.1016/S0040-4039(97)00973-8

Calcium chloride is an efficient additive for promoting the release of short peptide models anchored with Dpr(Phoc) linker to hydrophilic solid-phase synthesis supports. It was shown to induce a moderate to marked (especially for C-terminal proline peptides) increase in the rate of alkaline hydrolytic cleavage, without epimerization at the C-terminal residue, while substantially reducing the hydroxide ion concentration. Base-promoted side-reactions are therefore expected to be slowed down.