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We developed an electrochemical method to measure the enzy-
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the functionalised multi-walled carbon nanotube modified elec-
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and the amperometric measurements had high sensitivity and
wide linear dynamic range in detection of NADH. The amperomet-
ric measurements were successfully applied to determine the ini-
tial reaction rate, Michaelis constant and other kinetic
parameters of the ADH reaction system. We also found that both
Al3+ and Al13 exhibited inhibitory effect on the enzymatic activity
of ADH and the inhibition of Al13 is stronger. Fluorescence and cir-
cular dichroism spectra showed that Al3+ and Al13 could induce
conformational changes of ADH and NADH. However, more exten-
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Acknowledgements
The project is supported by the National Natural Science Foun-
dation of China (Grant No. 20875047), the research funding of Min-
istry of Water Resources (201201018) and the Priority Academic
Program Development of Jiangsu Higher Education Institutions.
XW acknowledges the financial support from the National Natu-
ral Science Foundation of China (21103016) and the Research Fund
for the Doctoral Program of Higher Education of China
(20100185120023).
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NADH
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