IMMOBILIZED ENZYME KINETIC STUDY OF D-GLUCOSE MUTAROTATION BY FLOW INJECTION ANALYSIS.

Article abstract of DOI:10.1021/ac00145a007

A novel analytical application of immobilized enzymes is presented in which the mutarotation kinetics of D-glucose are studied. A glucose oxidase single bead string reactor is combined with the Trinder reaction in a flow injection system. The apparatus is used to monitor the mutarotation of D-glucose in a batch reactor. The mutarotation coefficients obtained are in good agreement with the literature values. Phosphate ion is shown to catalyze the reaction and the mutarotation coefficient is directly proportional to its concentration. The selectivity of the enzyme reactor is also demonstrated.