Isolation and Some Properties of Sorbitol Oxidase from Streptomyces sp. H-7775

Article abstract of DOI:10.1271/bbb.61.1699

A sorbitol oxidase (SOX) was found in the cell-free extract of a strain isolated from soil. The strain was classified and designated as Streptomyces sp. H-7775. SOX is constitutively expressed in the cell. The molecular weight of SOX that purified from the cell-free extract was 45,000. The optimum pH and the K_m for sorbitol were 6.5-7.5 and 0.26 mM, respectively. The prosthetic group was a covalently bound FAD. SOX catalyzed oxidation of D-sorbitol to glucose and hydrogen peroxide without any requirements of exogenous cofactors. SOX did not react with glucose, a reaction product of D-sorbitol. This feature is useful in its application for diagnosis.

Full text of DOI:10.1271/bbb.61.1699

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Isolation and Some Properties of Sorbitol Oxidase
from Stveptomyces sp. H-7775
Kazumi Hiragaa, Mitsunori Kitazawaa, Norihisa Kanekoa & Kohei Odaa
a Department of Applied Biology, Faculty of Textile Science, Kyoto Institute of
Technology, Sakyo-ku, Kyoto 606, Japan
Published online: 12 Jun 2014.
To cite this article: Kazumi Hiraga, Mitsunori Kitazawa, Norihisa Kaneko & Kohei Oda (1997) Isolation and Some Properties
of Sorbitol Oxidase from Stveptomyces sp. H-7775, Bioscience, Biotechnology, and Biochemistry, 61:10, 1699-1704, DOI:
10.1271/bbb.61.1699
To link to this article: http://dx.doi.org/10.1271/bbb.61.1699
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