Bioorganic and Medicinal Chemistry (2021)
Update date:2022-08-16
Topics:
Shimizu, Takahiro
Takahashi, Norihito
Huber, Vincent J.
Asawa, Yasunobu
Ueda, Hiroki
Yoshimori, Atsushi
Muramatsu, Yukiko
Seimiya, Hiroyuki
Kouji, Hiroyuki
Nakamura, Hiroyuki
Oguri, Hiroki
Inspired by the privileged molecular skeletons of 14- and 15-membered antibiotics, we adopted a relatively unexplored synthetic approach that exploits alkaloidal macrocyclic scaffolds to generate modulators of protein–protein interactions (PPIs). As mimetics of hot-spot residues in the α-helices responsible for the transcriptional regulation, three hydrophobic sidechains were displayed on each of the four distinct macrocyclic scaffolds generating diversity of their spatial arrangements. Modular assembly of the building blocks followed by ring-closing olefin metathesis reaction and subsequent hydrogenation allowed concise and divergent synthesis of scaffolds 1–4. The 14-membered alkaloidal macrocycles 2–4 demonstrated similar inhibition of hypoxia-inducible factor (HIF)-1α transcriptional activities (IC50 between 8.7 and 10 μM), and 4 demonstrated the most potent inhibition of cell proliferation in vitro (IC50 = 12 μM against HTC116 colon cancer cell line). A docking model suggested that 4 could mimic the LLxxL motif in HIF-1α, in which the three sidechains are capable of matching the spatial arrangements of the protein hot-spot residues. Unlike most of the stapled peptides, the 14-membered alkaloidal scaffold has a similar size to the α-helix backbone and does not require additional atoms to induce α-helix mimetic structure. These experimental results underscore the potential of alkaloidal macrocyclic scaffolds featuring flexibly customizable skeletal, stereochemical, substitutional, and conformational properties for the development of non-peptidyl PPI modulators targeting α-helix-forming consensus sequences responsible for the transcriptional regulation.
Zipont chem(wuhan）Tech co.，Ltd
Contact:+86-27-87587198
Address:wuhan
Contact:86-575-86132822，86-575-86085355
Address:No.418 Dadao West Road,Qixing Street,Xinchang, Zhejiang Province, China.
CGeneTech (Suzhou, China) Co., Ltd.
Contact:+86-512-62956962
Address:Room 101，Bld C11,218 Xinghu Rd.,Suzhou industrial Park
website:http://www.jairadhesales.com
Contact:0091-79-26431096
Address:309 Harikrupa Tower,Nr old Sharda Mandir Char Rasta,Ellisbridge
Shijiazhuang Frontierchem Co., Ltd.
Contact:+86-311-89271196
Address:4-4-202 No.15 Biandian Street,Shijiazhuang
Doi:10.1246/cl.200056
(2020)Doi:10.1248/cpb.54.1715
(2006)Doi:10.1248/cpb.46.1810
(1998)Doi:10.1021/ja00803a070
(1973)Doi:10.1021/jm050915u
(2006)Doi:10.1134/S1070428006040075
(2006)
