A phosphate bound universal linker for DNA synthesis

Article abstract of DOI:10.1080/07328319908044845

A uridine-based linker immobilized onto polystyrene beads at the 5' terminus via a phosphodiester group and then used as a universal DNA synthesis support gives post synthesis DNA cleavage in 8 hrs or less without alkali metal salts. DNA produced with the new support was analyzed by HPLC, MALDI mass spectroscopy and PAGE. Each analysis showed DNA of equivalent quality to that produced with standard CPG supports, without contaminating materials resulting from linker or support backbone decomposition.

Full text of DOI:10.1080/07328319908044845

Nucleosides and Nucleotides
ISSN: 0732-8311 (Print) (Online) Journal homepage: http://www.tandfonline.com/loi/lncn19
A Phosphate Bound Universal Linker for DNA
Synthesis
Matthew H. Lyttle , Daren J. Dick , Derek Hudson & Ronald M. Cook
To cite this article: Matthew H. Lyttle , Daren J. Dick , Derek Hudson & Ronald M. Cook (1999)
A Phosphate Bound Universal Linker for DNA Synthesis, Nucleosides and Nucleotides, 18:8,
1809-1824, DOI: 10.1080/07328319908044845
To link to this article: http://dx.doi.org/10.1080/07328319908044845
Published online: 04 Oct 2006.
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Date: 08 November 2015, At: 01:59