Reconstitution of Apomyoglobin with Bile Pigments

Article abstract of DOI:10.1007/BF00808953

Apomyoglobin was reconstituted with bile pigments of the verdinoid, 2,3-dihydroverdinoid, pterobilinoid, and violinoid type.Absorption and circular dichroism data as well as formation constants of the complexes were measured.From these results it was concluded that chromophore binding and induced chirality of these pigments are mainly governed by a lipophilic region opposite to the propionic side chain(s) and the asymmetric position of the hydrogen bonding acceptors of the propionic acid side chain(s) at the entrance of the protein pocket.