Journal of Pharmaceutical Sciences p. 711 - 713 (1986)
Update date:2022-08-17
Topics:
Kinoshita
Nakamaru
Tanaka
Tohira
Sawada
A simple enzyme immunoassay for the determination of captopril was developed. A specific antibody for captopril was produced in rabbits that were immunized with a hapten-bovine immunoglobulin G conjugate, which was prepared by using 4-(maleimidomethyl)cyclohexane carboxylic acid as a spacer group. The limit of detection in plasma is 0.5 ng/mL. The assay has an adequate specificity, so isolation of captopril is unnecessary.
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