Steroids p. 47 - 56 (1984)
Update date:2022-08-29
Topics:
Tohma, Masahiko
Wajima, Hiromi
Mahara, Reijiro
Korosawa, Takao
Makino, Isao
The glycine conjugate of 3β-hydroxy-5-cholen-24-oic acid and its sulfate labeled with deuterium at the C-2, -4 and -23 positions were synthesized.A highly sensitive and specific quantitative assay of the bile acid has been developed by selected ion monitoring in gas chromatography-mass spectrometry of the methyl ester trimethylsilyl ether derivatives using the deuterium labeled conjugates as internal standards.Calibration curves for the bile acid and its sulfate exhibited a linear relationship over the range of 0.01-100 μg/ml in human serum.
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