THE EFFECT OF METAL ION COMPLEX FORMATION ON ACIDIC DEPURINATION OF 2'-DEOXYADENOSINE AND 2'-DEOXYGUANOSINE

Article abstract of DOI:10.1016/S0040-4020(01)89253-X

The substitution inert N7-(dien)Pt(II) complex of 2'-deoxyguanosine has been shown to undergo acidic depurination 200 times less readily than the uncomplexed nucleoside, whereas the corresponding N1- and N7-complexes of 2'-deoxyadenosine are depurinated almost as rapidly as the nucleoside itself.These observations have been compared to the influences that several substitution labile metal ions exerted on the rate of depurination.Accordingly, the effects of (dien)Pd(II), Co(II), Ni(II), Cu(II), Zn(II) and Cd(II) ions on the acidic hydrolysis of 2'-deoxyadenosine and 2'-deoxyguanosine have been accounted for by competitive attachment of protons and metal ions to the N1 and N7 sites.The applicability of metal ions in chemical DNA sequencing is briefly discussed.