The antibacterial properties of these analogues are tested by exposing them to different bacterial strains. Researchers use techniques such as agar diffusion assays or broth microdilution methods to determine the minimum inhibitory concentration (MIC), which is the lowest concentration at which the compound inhibits bacterial growth.
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1,7-bis(4-hydroxy-3-methoxyphenyl)-4-(2-(p-tolyl)hydrazono)hepta-1,6-diene-3,5-dione
2,6-bis(6-(4-chlorophenyl)-1,2,4-triazolo[3,4-b][1,3,4]thiadiazol-3-yl)pyridine
