The enzyme is added to the reaction mixture, and the reaction is allowed to proceed under controlled conditions. The enzyme facilitates the ring-closing reaction, leading to the formation of the lactone ring. The specific conditions, including temperature, pH, and reaction time, will depend on the enzyme and substrate.
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γ-phenyl-γ-butyrolactone
(R)-3,3,3-Trifluoro-2-methoxy-2-phenyl-propionic acid (R)-6,7-dimethoxy-2-methyl-1,2,3,4-tetrahydro-isoquinolin-4-yl ester
(+)-(1S,2S,3S)-2,3-bis<(benzyloxy)methyl>-1-cyclobutyl acetate
(4S-cis)-5-[2'-(hydroxymethyl)phenyl]-2,2-dimethyl-1,3-dioxolane-4-methanol
1,3-dipropionyloxy-2-(2,4-difluorophenyl)-2-propanol
(R)-ethyl 1,1-diethoxyethyl{[(diphenylmethylene)-amino]methyl}phosphinate
3-Pyrrolidinecarboxylic acid, methyl ester, (3S)- (9CI)
