SAR optimization

Synthesis Antagonist Lipophilicity Yield In vitro Selectivity Bioassay Derivatives Molecular docking Lead compound Apoptosis Analog Mass spectrometry (MS) IC50 Pharmacophore Kinetics Enantiomers Cytotoxicity binding affinity Thiourea HPLC (high-performance liquid chromatography) NMR (nuclear magnetic resonance) Enzyme inhibition in vivo Inhibitor Purification In vitro Testing In Vivo Testing Biological Evaluation Docking Studies Antitumor Activity Structure-Activity Relationship (SAR) Pharmacokinetics Substrate Thiazole Dose-Response Curve Topoisomerase Inhibition Flow Cytometry Electron-Withdrawing/Donating Groups Toxicity Profile In vitro assay Diamine Alkylating Agents Prodrug Protease inhibitors Receptor binding assay Antiproliferative agents Synthetic derivatives Allosteric site Catalytic site