The DNA template is used as a template for in vitro transcription, with the RNA polymerase enzyme and the necessary nucleotide triphosphates, including the unnatural base pairs. As the RNA polymerase reads the template, it incorporates the unnatural base pairs into the growing RNA strand.
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3-(5-O-(4,4'-dimethoxytrityl)-β-D-ribofuranosyl)-5-iodo-2-oxo(1H)pyridine
C35H45N8Ni2O9(1+)*ClO4(1-)
α-D-glucopyranosyl-(1→4)-α-D-glucopyranosyl-(1→4)-α-D-sorbitol
Acetic acid (2R,3S,5R)-2-acetoxymethyl-5-(4-benzyloxy-5-methyl-2-oxo-2H-pyrimidin-1-yl)-tetrahydro-furan-3-yl ester
