499-61-6Relevant articles and documents
Hydrochloric acid isopropyl adrenergic preparation method
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Paragraph 0014-0016; 0021-0023; 0028-0030, (2019/03/31)
The invention discloses a hydrochloric acid isopropyl adrenergic preparation method, comprises the following steps: through the catechol with glycine in the role of the catalyst [...] reaction to obtain 2 - amino - 1 - (3, 4 - dihydroxy-phenyl) - ethyl ketone; through the 2 - amino - 1 - (3, 4 - dihydroxy-phenyl) - ethanone with 2 - chloro propane reaction preparation isopropyl adrenergic ketone bodies hydrochloride; through the isopropyl adrenergic ketone bodies hydrochloride in the catalytic reduction of the palladium-carbon under the action of hydrochloric acid isopropyl adrenergic. Selects the glycin, zinc chloride the reaction system, which not only reduces the costs of environmental protection, also improves the reaction yield, is favorable for industrial production; mild reaction conditions, less catalyst levels, the process is simple; it has obvious economic and environmental benefits.
Purification and characterization of a novel carbonyl reductase involved in oxidoreduction of aromatic β-amino ketones/alcohols
He, Shengbin,Wang, Zhenshou,Zou, Yang,Chen, Shaofang,Xu, Xiaoping
, p. 1107 - 1112 (2014/06/10)
Aromatic β-amino ketones/alcohols such as adrenalone play an important role in some stereoselective synthesis of pharmaceuticals. Unfortunately, the transformation of aromatic β-amino ketones to their chiral alcohols has been carried out chemically as no corresponding biocatalyst has been available. Here, a novel carbonyl reductase responsible for the reduction of adrenalone to (R)-(-)-epinephrine was identified and characterized from Kocuria rhizophila. This enzyme was purified to homogeneity by ammonium sulfate precipitation followed by ion-exchange column chromatography, hydrophobic chromatography and gel chromatography. The purified enzyme yielded pure (R)-enantiomer product with high activity and utilized NADH as the cofactor. The enzyme had special significance by showing selectivity for many aromatic β-amino ketones/alcohols such as 2-amino-acetophenone, 2-amino-4′- hydroxyacetophenone, isoproterenol and ephedrine. The maximum reaction rate (Vmax) and apparent Michaelis-Menten constant (Km) for adrenalone and NADH were 14.62 μmol/(min mg) protein and 0.189 mM, 11.66 μmol/(min mg) protein and 0.204 mM respectively. These properties ensure the enzyme a promising future for industrial application as a replacement of chemical synthesis of aromatic β-amino chiral alcohols.
Studies of the sclerotization of insect cuticle. The structure of a dimeric product formed by incubation of N-acetyldopamine with locust cuticle
Andersen,Jacobsen,Roepstorff
, p. 3249 - 3252 (2007/10/02)
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