5707-59-5Relevant academic research and scientific papers
The studies on the chemoenzymatic synthesis of 2-benzyl-3-butenoic acid
Borys, Filip,Paprocki, Daniel,Koszelewski, Dominik,Ostaszewski, Ryszard
, p. 6 - 9 (2018/06/01)
The studies on the chemoenzymatic synthesis of 2-benzyl-3-butenoic acid 3 were performed. The alkylation of dienolates derived from the crotonic and vinylacetic acids with different types of alkylating agents was examined. In all cases the inseparable mixture of isomeric acids 3 and 4 were obtained. Chemoselective enzymatic esterification of those mixtures provides ester 6 in 86% yield as a single product without double bond isomerization. None common method can be used for its hydrolysis since under basic conditions only isomeric acid 5 is formed. To overcome this limitation enzyme catalysed hydrolysis process was design. This protocol afforded isomerically pure acid 3 in 98% yield.
Exploration of Neutral Endopeptidase Active Site by a Series of New Thiol-Containing Inhibitors
Gomez-Monterrey, Isabel,Turcaud, Serge,Lucas, Evelyne,Bruetschy, Luce,Roques, Bernard P.,Fournie-Zaluski, Marie-Claude
, p. 87 - 94 (2007/10/02)
With the aim of characterizing the active site of the neutral endopeptidase and especially its putative S1 subsite, two series of new thiol inhibitors designed to interact with the S1, S'1, and S'2 subsites of the enzyme have been synthesized.These molecules correspond to the general formula HSCH(R1)CH(R2)CONHCH(R3)COOH (series I) and HSCH(R1)CH(R2)CONHCH(R3)CONHCH(R4)COOH (series II).Due to the synthetic pathway used, these inhibitors were obtained as mixtures of four stereoisomers.HPLC separation of the stereoisomers of 17 HSCHCH(CH2Ph)CONHCH(CH3)COOH allowed the stereochemical dependence of the inhibitory potency to be determined.The most active isomer 17b (IC50 = 3.6 nM) is assumed to have the S,S,S stereochemistry as deduced from both NMR and HPLC data.Although none of the inhibitors obtained were significantly more active than thiorphan, HSCH2CH(CH2Ph)CONHCH2COOH (IC50 = 4 nM), which interacts only with the S'1 and S'2 subsites of NEP, their enhanced hydrophobicity is expected to improve their pharmacokinetic properties.All these compounds displayed low affinities for ACE (IC50S > 1 μM).The determination of the IC50S of two inhibitors of series II for NEP and for a mutated enzyme in which Arg102 was replaced by Glu102 allowed their mode of binding to the active site of NEP to be characterized.The R2 and R3 chains fit the S'1-S'2 subsites, while the R4 group is probably located outside the active site.Taken together these results indicate that the R1 chain of these inhibitors creates no additional stabilizing interactions with the active site of NEP.Two hypotheses may account for this: there is no hydrophophobic S1 subsite in NEP or the inhibitors have structures which are too constrained for optimized interactions with the active site.
