A phenolic antioxidant from the Pacific oyster (Crassostrea gigas) inhibits oxidation of cultured human hepatocytes mediated by DIPHENYL-1-PYRENYLPHOSPHINE (cas 18995-02-3)

Add time:07/23/2019    Source:sciencedirect.com

3,5-Dihydroxy-4-methoxybenzyl alcohol (DHMBA), an antioxidant isolated from the Pacific oyster (Crassostrea gigas), was studied in a cell-based fluorometric antioxidant assay using human hepatocyte-derived cells (C3A) and diphenyl-1-pyrenylphosphine (DPPP) as a fluorescent probe. In comparison with two hydrophilic antioxidants, DHMBA showed the stronger inhibition of DPPP-mediated fluorescence than chlorogenic acid and l-ascorbic acid: at a concentration of 320 μM of DPPP, the inhibition was 26.4 ± 2.6%, 11.1 ± 1.2%, and 0 ± 2.0% for DHMBA, chlorogenic acid, and l-ascorbic acid, respectively (mean ± SD, n = 4). Their relative oxygen radical absorbance capacities (ORAC) were dissociated with their cell-based antioxidant activities: 1.47 ± 0.40, 4.57 ± 0.30, and 0.53 ± 0.13 μmol TE/μmol for DHMBA, chlorogenic acid, and l-ascorbic acid, respectively (mean ± SD, n = 4). The amphiphilicity of DHMBA was better than chlorogenic acid and l-ascorbic acid might underlie this dissociation. Since the C3A cells are human hepatoma-derived cells, DHMBA might be useful in the prevention and treatment of liver diseases by involving an oxidation process.

We also recommend Trading Suppliers and Manufacturers of DIPHENYL-1-PYRENYLPHOSPHINE (cas 18995-02-3). Pls Click Website Link as below: cas 18995-02-3 suppliers

Prev:A novel fluorescent probe DIPHENYL-1-PYRENYLPHOSPHINE (cas 18995-02-3) to follow lipid peroxidation in cell membranes
Next:A novel method of following oxidation of low-density lipoprotein using a sensitive fluorescent probe, DIPHENYL-1-PYRENYLPHOSPHINE (cas 18995-02-3))