The chromatographic separation of uroporphyrin I and III octamethyl esters

Add time:07/19/2019    Source:sciencedirect.com

Uroporphyrins I and III have been separated by high performance liquid chromatography on a microporasil column using n-heptane:glacial acetic acid:acetone:water (90:60:30:0.05 by volume). By using uro [3H]porphyrin I and uro[14C]porphyrin III it was shown that less than 2% cross contamination occurred during the chromatographic separation. The same system may be used to separate uro through protoporphyrins (as their esters) by simply increasing the acetone concentration.

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