Simultaneous determination of itraconazole, HYDROXY ITRACONAZOLE (cas 112559-91-8), keto itraconazole and N-desalkyl itraconazole concentration in human plasma using liquid chromatography with tandem mass spectrometry

Add time:07/23/2019    Source:sciencedirect.com

A high-performance liquid chromatography tandem mass spectrometry (LC–MS/MS) assay was developed and validated for simultaneous determination of itraconazole (ITZ), hydroxy-itraconazole (OH-ITZ), keto-itraconazole (keto-ITZ) and N-desalkyl itraconazole (ND-ITZ) concentration in human plasma. One hundred and fifty microliters of human plasma were extracted using a solid-supported liquid extraction (SLE) method and the final extracts were analyzed using reverse-phase chromatography and positive electrospray ionization mass spectrometry. The standard curve range is 5–2500 ng/mL for ITZ and OH-ITZ and 0.4–200 ng/mL for keto-ITZ and ND-ITZ. The curve was fitted to a 1/x2 weighted linear regression model for all analytes. The precision and accuracy of the LC–MS/MS assay based on the five analytical quality control (QC) levels were well within the acceptance criteria from both FDA and EMA guidance for bioanalytical method validation. Average extraction recovery was 97.4% for ITZ, 112.9% for OH-ITZ, 103.4% for keto-ITZ, and 102.3% for ND-ITZ across their respective curve range. Matrix factor was close to 1.0 at both high and low QC levels of all 4 analytes, which indicates minimal ion suppression or enhancement in our validated assay. Itraconazole and all three metabolites are stable in human plasma for 145 days stored at −70 °C freezers. The validated assay was successfully applied to a clinical study, which has a drug-drug interaction (DDI) arm using ITZ as a cytochrome P450, family 3, subfamily A (CYP3A) inhibitor.

We also recommend Trading Suppliers and Manufacturers of HYDROXY ITRACONAZOLE (cas 112559-91-8). Pls Click Website Link as below: cas 112559-91-8 suppliers

Prev:Simultaneous determination of voriconazole, posaconazole, itraconazole and hydroxy-itraconazole in human plasma using LCMS/MS
Next:Ultra-performance liquid chromatography electrospray ionization–tandem mass spectrometry method for the simultaneous determination of itraconazole and HYDROXY ITRACONAZOLE (cas 112559-91-8) in human plasma)