The role of Bach1 in the induction of heme oxygenase by tin mesoporphyrin (cas 106344-20-1)

Add time:07/23/2019    Source:sciencedirect.com

Tin mesoporphyrin (SnMP), a competitive heme oxygenase (HO) inhibitor, also induces HO-1 mRNA and protein expression by a mechanism that is not fully understood. We examined whether the induction by SnMP is mediated by a de-repression of Bach1, a transcription factor that suppresses the HO-1 gene. Incubation of NIH3T3-HO-1-luc cells with SnMP attenuated HO activity with a concomitant increase in HO-1 mRNA and protein and a decrease in Bach1 and HO-2 proteins, which was not due to transcriptional down-regulation, but accelerated protein decay. Similarly, HO-1 protein degradation was increased by SnMP, despite of an elevation in HO-1 transcription. Transfection of Bach1 shRNA in Hepa cells raised basal HO-1 expression significantly, and SnMP treatment further increased HO-1 mRNA. In conclusion, SnMP induces HO-1 expression not only by de-repressing the HO-1 promoter by binding Bach1, but also by accelerating Bach1 degradation.

We also recommend Trading Suppliers and Manufacturers of tin mesoporphyrin (cas 106344-20-1). Pls Click Website Link as below: cas 106344-20-1 suppliers

Prev:Microstructural correlations to micromechanical properties of polyamide-6/low density polyethylene-grafted-maleic anhydride/nanoclay ternary nanocomposites
Next:Solvent effects on the absorption and fluorescence characteristics of tin(IV) mesoporphyrin)