Quantitative dünnschichtchromatographische bestimmung des makrolidantibiotikums turimycin h und einiger abba uprodukte durch densitometrische in-situ-messung mit dem bildanalysegerät “quantimet 720”

Add time:07/23/2019    Source:sciencedirect.com

Quantitative thin-layer chromatographic determination of the macrolide antibiotic turimycin H and some of its degradation products by densitometric in situ measurement with the image analyser “Quantimet 720”A method for the quantitative determination of the macrolide antibiotic complex turimycin H, its components turimycin H2, H3 and H5, as well as the degradation products 4″-deacylturimycin H and demycarosylturimycin H, is described. Thin-layer chromatography is used for the separation of the mixtures. The image analyser “Quantimet 720” is employed for densitometric in situ quantitation. The reproducibility of evaluation of coloured spots on thin-layer chromatograms by image analysis is studied. Spotting errors, conditions of visualiztion and the sensitivity of the detector (vidicon tube) were responsible for the variance of repeated determinations. The method is demonstrated by analysis of some synthetic mixture as well as turimycin complexes obtained from different fermentation batches.

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