Detection of 2-keto-3-deoxyoctonate (cas 1069-03-0) in endotoxins isolated from six reference strains of the Bacteroides fragilis group

Add time:07/26/2019    Source:sciencedirect.com

1.1. Endotoxins isolated from six serotype specific reference strains of the Bacteroides fragilis group were dephosphorylated by treatment with aqueous 50% hydrofluoric acid.2.2. Mild acidic hydrolysis of the dephosphorylated endotoxins released 2-keto-3-deoxyaldonic acid, the presence of which was demonstrated by the colorimetric thiobarbituric acid assay (TBA).3.3. Thinlayer chromatography of the dephosphorylated lipopolysaccharide of B. fragilis IPL E 323 (serotype E2, after acidic hydrolysis, revealed a TBA-positive substance with the same Rf-value as authentical 2-keto-3-deoxyoctolusonic acid (KDO).4.4. Quantification of2-keto-3-deoxyoctonate (cas 1069-03-0) in the lipopolysaccharide of B. fragilis IPL E 323 by means of the TBA resulted in a KDO content of 15 nM mg−1 lipopolysaccharide.

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