A new and improved microassay to determine 2-keto-3-deoxyoctonate (cas 1069-03-0) in lipopolysaccharide of gram-negative bacteria

Add time:07/29/2019    Source:sciencedirect.com

A procedure is described to determine 2-keto-3-deoxyoctonate (cas 1069-03-0) (KDO) present in lipopolysaccharide (LPS) of gram-negative bacteria. The method involves the treatment of LPS with 0.2 n H2SO4 at 100°C for 30 min to release KDO, followed by its reaction with periodic acid, sodium arsenite, and thiobarbituric acid. The red chromophore thus formed is kept in solution at room temperature by adding dimethylsulfoxide to the reaction mixture. The final color is stable for days at room temperature and facilitates accurate determination of KDO in microgram quantities. KDO contents of cell surface antigens and glycolipids from gram-negative bacteria are presented as illustrations of the accuracy and sensitivity of the assay.

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