Isolation and Characterization of Acetyl-CoA Synthetase from Etiolated Radish Seedlings

Add time:07/25/2019    Source:sciencedirect.com

SummaryAcetyl-CoA synthetase (ACS; E. C. 6.2.1.1.) is a key enzyme in plastids of higher plants which provides the starting substrate acetyl-CoA for de novo fatty acid biosynthesis. The ACS enzyme activities, measured in leaves and chloroplasts of different plants, are described. The enzyme was purified 183-fold from etiolated radish cotyledons to a final specific activity of 193 nmol min−1mg−1 protein. The properties, the substrate specifity and the Michaelis-Menten constants (Km for ATP, acetate and coenzyme A) of the purified enzyme were determined. The ACS exhibits a high substrate specifity for acetate as compared to other organic acids (e.g. propionic, acrylic or butyric acid).

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