Determination of 3-mercaptolactic acid by amino acid analyzer after aminoethylation

Add time:07/27/2019    Source:sciencedirect.com

A quantitative determination of 3-mercaptolactic acid was performed after its conversion into S-aminoethylmercaptolactic acid by reacting with excess of 2-bromoethylamine. S-aminoethylmercaptolactic acid was quantitated by an amino acid analyzer. Other thiols were shown not to interfere with the determination of 3-mercaptolactic acid. The sensitivity of the method was at the nanomoles level. The application of the method to the determination of 3-mercaptolactic acid in human urine is also reported.

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