High-performance liquid chromatographic analysis of arachidonic acid metabolites by pre-column derivatization using 9-ANTHRYLDIAZOMETHANE (cas 10401-59-9)
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Add time:08/03/2019 Source:sciencedirect.com
Arachidonic acid (AA) metabolites produced by washed human platelets and rat macrophages were analyzed by high-performance liquid chromatography (HPLC) using a pre-column derivatization method. The reagent, 9-anthryldiazomethane, used in this study and AA metabolites derivatized by the reagent were purified by gel permeation chromatography (PG-peak C column), prior to normal-phase HPLC analysis. A sample containing eleven derivatives (12-, 15- and 5-hydroxyeicosatetetraenoic acid, 12-l-heptadecatrienoic acid, leukotriene B4, prostaglandins B2, D2, E2 and F2α, thromboxane B2 and 6-keto-prostaglandin F1α) was separated on a normal-phase column (PG-pak B); the detection limit is better than 100 pg for all components.
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