Potential interference of hydrogen peroxide in the 2,2′-bicinchoninic acid protein assay

Add time:08/05/2019    Source:sciencedirect.com

At a concentration of 20–800 nmol0.1 ml hydrogen peroxide instantly reacts with a 2,2′-bicinchoninic acid copper color reagent. It also reacts with a reformulated reagent at pH 7 but the color develops less rapidly. While the effect may interfere with protein estimations at alkaline pH, the effect of pH 7 may be used to determine the possible extent of hydrogen peroxide interference after treatment with catalase.

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