pH and buffering in the bicinchoninic acid (4,4′-dicarboxy-2,2′-biquinoline) protein assay

Add time:08/06/2019    Source:sciencedirect.com

The HCO3−/CO32− buffer used in the bicinchoninic acid (BCA) protein assay has only weak buffering capacity at the recommended pH (11.25). Consequently the assay is rather sensitive to interference from effectively acid or alkaline samples, particularly in the micro method. Adjustment of pH in these alkaline solutions of high [Na+] is complicated by Na+ errors on the pH electrode. Hence it is recommended to prepare the buffers from known amounts of NaHCO3 and Na2CO3, and to reduce the pH to around 10.7; this offers much better buffering capacity with only a limited reduction in color development.

We also recommend Trading Suppliers and Manufacturers of 2,2'-BICINCHONINIC ACID (cas 1245-13-2). Pls Click Website Link as below: cas 1245-13-2 suppliers

Prev:Potential interference of hydrogen peroxide in the 2,2′-bicinchoninic acid protein assay
Next:Potassium Penicillin V)