obtusifoliol (cas 16910-32-0) 14α-Demethylase (CYP51) Antisense Arabidopsis Shows Slow Growth and Long Life
-
Add time:08/08/2019 Source:sciencedirect.com
obtusifoliol (cas 16910-32-0) 14α-demethylase is a plant orthologue of sterol 14α-demethylase (CYP51) essential in sterol biosynthesis. We have prepared CYP51 antisense Arabidopsis in order to shed light on the sterol and steroid hormone biosynthesis in plants. Arabidopsis putative CYP51 cDNA (AtCYP51) was obtained from Arabidopsis expressed sequence tag (EST) library and its function was examined in a yeast lanosterol 14α-demethylase (Erg11) deficient mutant. A recombinant AtCYP51 protein fused with a yeast Erg11 signal-anchor peptide was able to complement the erg11 mutation, which confirmed AtCYP51 to be a functional sterol 14α-demethylase. AtCYP51 was then used to generate transgenic Arabidopsis by transforming with pBI vector harboring AtCYP51 in the antisense direction under CaMV35S promoter. The resulting transgenic plants were decreased in accumulation of AtCYP51 mRNA and increased in the amount of endogenous obtusifoliol. They showed a semidwarf phenotype in the early growth stage and a longer life span than control plants. This newly found phenotype is different from previously characterized brassinosteroid (BR)-deficient campesterol biosynthesis mutants.
We also recommend Trading Suppliers and Manufacturers of obtusifoliol (cas 16910-32-0). Pls Click Website Link as below: cas 16910-32-0 suppliers
Prev:Plant sterol biosynthesis: 7-oxo-obtusifoliol (cas 16910-32-0) analogues as potential selective inhibitors of cytochrome P-450 dependent obtusifoliol (cas 16910-32-0) 14α-demethylase
Next:Stereochemistry of C-4 demethylation during conversion of obtusifoliol (cas 16910-32-0) into poriferasterol by Ochromonas malhamensis) - 【Back】【Close 】【Print】【Add to favorite 】
- Related Information
- Plant sterol biosynthesis: Novel potent and selective inhibitors of cytochrome P450-dependent obtusifoliol (cas 16910-32-0) 14α-methyl demethylase08/11/2019
- The 14α-demethylation of obtusifoliol (cas 16910-32-0) by a cytochrome P-450 monooxygenase from higher plants microsomes08/10/2019
- Stereochemistry of C-4 demethylation during conversion of obtusifoliol (cas 16910-32-0) into poriferasterol by Ochromonas malhamensis08/09/2019
- Plant sterol biosynthesis: 7-oxo-obtusifoliol (cas 16910-32-0) analogues as potential selective inhibitors of cytochrome P-450 dependent obtusifoliol (cas 16910-32-0) 14α-demethylase08/07/2019
- obtusifoliol (cas 16910-32-0) and related steroids from the whole herb of Euphorbia chamaesyce08/06/2019
- Molecular Identification and Expression Analysis of GhCYP51G1 Gene, a Homologue of obtusifoliol (cas 16910-32-0)-14Alpha-Demethylase Gene, from Upland Cotton08/05/2019
- obtusifoliol (cas 16910-32-0) related steroids from Euphorbia sogdiana with cell growth inhibitory activity and apoptotic effects on breast cancer cells (MCF-7 and MDA-MB231)08/04/2019