Characterization of esterases in malathion-resistant and susceptible strains of the pteromalid parasitoid Anisopteromalus calandrae

Add time:08/14/2019    Source:sciencedirect.com

General esterase, malathion-specific carboxylesterase, phosphotriesterase, glutathione S-transferase, cytochrome P-450-dependent monooxygenase activity, and target site sensitivity were compared in malathion-resistant (R) and malathion-susceptible (S) strains of the parasitoid Anisopteromalus calandrae (Howard) (Hymenoptera: Pteromalidae). Activity against α-naphthyl acetate was not significantly different in male and female wasps for either strain. General esterase activity ranged from 1.2-fold to 2.5-fold higher in the R strain compared with the S strain, but these differences between strains were not consistent. Based on Vmax/Km ratios estimated for a number of analogs of four substrates (α-naphthyl acetate, β-naphthyl acetate, 4-methylumbelliferyl acetate, and p-nitrophenyl acetate) there was no evidence that general esterase activity was elevated or reduced in the R strain. Malathion-specific carboxylesterase (MCE) activity, determined by using 2,3-14C-malathion as substrate, was 10- to 30-fold higher in the R strain compared with that in the S strain. The MCE has a pH optima at about pH 7, is cytosolic, and is labile upon storage at −80°C. MCE activity could be recovered from native 10% PAGE gels and IEF–PAGE gels (pI=5.2), but the peak of MCE activity also contained the major peak of activity against α-naphthyl acetate. There was no evidence for major involvement of phosphotriesterase, glutathione S-transferase, monooxygenase, or altered acetylcholinesterase in the resistance. These data suggest that an increased activity of a MCE in the R strain is the probable major mechanism conferring resistance to malathion in A. calandrae. This study provides the first characterization of a biochemical resistance mechanism in a parasitoid with a high level of resistance to an organophosphate insecticide.

We also recommend Trading Suppliers and Manufacturers of Malathion β-Monoacid (cas 1642-51-9). Pls Click Website Link as below: cas 1642-51-9 suppliers

Prev:Tissue specific esterase and malathion carboxylesterase activity in larvae of malathion-resistant Plodia interpunctella (Hübner) (Lepidoptera: Pyralidae)
Next:The biochemical basis of tolerance to malathion in Rhodnius prolixus)