Metabolism of [3H]gibberellin A5 and [2H]gibberellin A5 in cell suspension cultures of Prunus persica

Add time:08/07/2019    Source:sciencedirect.com

Metabolism of the native gibberellin A5 (GA5), fed as either [1-3H] GA5 (3.1 Ci/mmol) or [17,17-2H]GA5, was investigated in cell suspension cultures of peach (Prunus persica) after 48 hr of incubation. Extractable radioactivity in free GA metabolites was 57.5%, with 21.6% in the putative GA glucosyl conjugates fraction.Tentative identifications, based on comparison with authentic standard GAs after sequential chromatography on silica gel column → gradient-eluted C18 reversed phase HPLC-radiocounting (RC) → isocratic-eluted C18 reversed phase HPLC-RC [→ Nucleosil N(Me)2 HPLC-RC, when necessary], showed that only 13.9% of the extractable radioactivity remained as [3H]GA5, with 2.7% as [3H]GA5 glucosyl ether (GA5-G) and 4.3% as [3H]GA, glucosyl ester (GA5-GE). [3H]GA5 was converted to the free GAs, GA1, GA3, GA6, GA8 and GA22, and the putative GA glucosyl conjugates, GA1-0(3)-G, GA1-0(13)-G, GA3-0(3)-G, GA3-0(13)-G, GA5-G, GA5-GE, GA6-GE, GA8-0(2)-G and GA8-0(?)-G. From the feeds of [17,17-2H]GA5 the presence of GA1, GA3, GA5, GA6, GA8, GA22, GA5-G and GA5-GE was further confirmed by GC-SIM, using five characteristic ions and Kovats Retention Indices.

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