Metabolism of [3H]gibberellin A5 in developing pharbitis nil seeds

Add time:08/08/2019    Source:sciencedirect.com

The native gibberellin A5 (GA5), as [1-3H]GA5 (3.2 Ci/mmol) was fed to seed capsules (0.58 μCi/capsule) of Pharbitis nil cv Violet at the 2-week stage of development, and its metabolism in the seeds was investigated after 43 hr. Extractable radioactivity in free GA metabolites was 38%, with 56% in GA glucosyl conjugate-like substances. Only 2.5% of the extractable radioactivity remained as [3H]GA5. Tentative identifications, based on comparisons with authentic standards after sequential chromatography on silica gel partition column → gradient-eluted C18 HPLC → isocratic-eluted C18 HPLC-radiocounting (RC), showed that [3H]GA5 was converted to at least six free GAs, GA1, GA3, GA6, GA8, GA22, GA29, a GA5 methyl ester-like metabolite, and at least twelve GA glucosyl conjugate-like substances, GA5-glucoside (GA5-G), GA5-glucosyl ester (GA5-GE), GA1-O(3)-G, GA1-O(13)-G, GA1-GE, GA3-O(3)-G, GA3-O(13)-G, GA3-GE, GA6-G or GE, GA8-O(2)-G, GA22-G or GE and GA29-O(2)-G. After lower specific activity feeds of [1,2-3H]GA5 (74 mCi/mmol; 0.1 μCi/capsule) at approximately the same stage of development, the presence of GA1, GA3, GA5, GA6, GA8 and GA29 was further confirmed by sequential (after C18 HPLC-RC) capillary gas chromatography-selected ion monitoring (GC-SIM), using six characteristic ions. However, for GA22 only a trace of the parent ion was present at the appropriate retention time.

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