Gas-Liquid Chromatographic Determination of Pantothenates and Panthenol (cas 16485-10-2)
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Add time:08/13/2019 Source:sciencedirect.com
A procedure has been developed for the identification and quantitative determination of the pantothenates and panthenol by GLC. The pantothenate salts were converted to an ethyl ester by treatment with anhydrous ethanolic hydrogen chloride. The acetate derivatives of the pantothenic acid ethyl ester and panthenol were prepared by acetylation of the hydroxyl groups with a pyridineracetic anhydride mixture. The excess reagents were removed and the oily residue was taken up in chloroform and injected on a 2.438-m. (8-ft.) × 4-mm. column packed with 2% neopentyl glycol sebacate (NPGSb). Trimethylsilyl and trifluoroacetate derivatives of the pantothenates and panthenol were also analyzed by gas chromatography. The procedure is very sensitive; as little as 0.25 mcg. can be detected. Structures of the acetate derivatives were confirmed by IR and NMR spectroscopy.
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