Establishing a novel assay system for measuring renin concentration using cost effective recombinant ovine angiotensinogen
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Add time:08/10/2019 Source:sciencedirect.com
BackgroundPlasma renin can predict future cardiovascular events as well as the prevalence of chronic renal disease in hypertensive subjects. Ovine angiotensinogen (oANG) is a better substrate for measuring renin concentration through activity assay. Recombinant oANG expressed in Escherichia coli cells can be utilized as the substrate while measuring plasma renin. We aim to establish an immunoassay for measuring renin concentration at picomolar level using recombinant oANG.
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