Comparison of thin-layer chromatography and overpressured layer chromatographic techniques for the separation of Ascorbigen A (cas 26676-89-1)nd 1′-methylascorbigen

Add time:07/21/2019    Source:sciencedirect.com

Simple and efficient methods are described for the separation of biologically active ascorbigen and 1′-methylascorbigen by normal-phase TLC, conventional as well as new personal overpressured layer chromatographic (OPLC) systems using the same eluent. Results obtained with these techniques were compared on the basis of the separation parameters with special emphasis on the presentation of the advantages of the automated personal OPLC system. For the characterization of the personal OPLC system dependence of average plate height and resolution on the external pressure, front velocity and development distance was examined on TLC and HPTLC sorbent layers using ascorbigen and 1′-methylascorbigen as model compounds.

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