Determination of gangliosides and sulfatide in human cerebrospinal fluid with a microimmunoaffinity technique

Add time:08/18/2019    Source:sciencedirect.com

An immunostaining procedure has been developed for the assay of the gangliotetraose gangliosides and sulfatide in cerebrospinal fluid. Gangliosides of the gangliotetraose series were individually determined with cholera toxin B-subunit (CT-B) and an anti CT-B monoclonal antibody after chromatography and sialidase hydrolysis to GM1 on high performance thin-layer plates. Sulfatide was determined by thin-layer chromatography using an anti-sulfatide antibody. The method was applied to normal cerebrospinal fluid from 20 adults and 30 children. The concentration of the gangliotetraose series gangliosides in adults varied from 100–300 nmol/l with a mean value of 230 ± 56 nmol/l. Corresponding values for sulfatide were 30–225 nmol/l and 140 ± 46 nmol/l. The values for gangliosides and sulfatide in children increased during development. The major gangliosides in cerebrospinal fluid of adults were GTlb and GDlb and in children GDla and GTlb.

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