PaperAn enzyme immunoassay of human polymorphonuclear leukocyte cathepsin G
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Add time:08/14/2019 Source:sciencedirect.com
An enzyme immunoassay has been developed for the quantitation of human polymorphonuclear leukocyte cathepsin G. The assay had a linear relationship over the range 0.23 – 4.7 nmol/l (6 – 125 ug/1) and a detection limit of 0.23 nmol/1(6 ug/1). Recovery in citrated plasma only occurred when the concentration was higher than 4.0 umol/l(110 mg/l). This effect could be overcome by diluting the plasma before adding the proteinase or by inactivating the proteinase before diluting it with plasma. The failure to detect cathepsin G in plasma was due to the plasma inhibitor alpha-2-macroglobulin masking the antigenic sites of the proteinase. Samples from several types of leukemia showed no detectable cathepsin G even when the total myeloid count was up to ten times the normal.
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