The effect of thiodiglycol and dithiothreitol on the alkaline hydrolysis products of certain amino acid phenylthiohydantoins☆

Add time:08/12/2019    Source:sciencedirect.com

The thiazolinone and phenylthiohydantoin derivatives of most amino acids can be hydrolyzed with alkaline dithionite to generate the free amino acid. The acidification of this hydrolysate with 3 n HCl containing thiodiglycol leads in the case of glutamic acid, glutamine, aspartic acid, asparagine, and S-carboxymethyleysteine to the generation of ninhydrin-reacting components having the chromatographic properties of other amino acids. The use of dithiothreitol instead of thiodiglycol appears to be more satisfactory in most instances.

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