Use of peptidyl-4-methoxy-2-naphthylamides to assay plasmin

Add time:08/12/2019    Source:sciencedirect.com

Several tripeptide amides of 4-methoxy-2-naphthylamide were synthesized and tested as possible substrates for human plasmin. These peptides contained arginine or lysine as the carboxyl terminus. One such amide, benzyloxycarbonyl-Ala-Ala-Lys-4-methoxy-2-naphthylamine (CBZ-Ala-Ala-Lys-MNA), was found to be a better substrate for plasmin than for other mammalian serine proteases. Measuring the release of 4-methoxy-2-naphthylamine fluorometrically or by colorimetry after coupling with fast blue B dye provided convenient assays for as little as 0.05 CTA unit of plasmin. Km and kcat values obtained were 0.90 mm and 0.7 sec−1, respectively. The assays were simple, sensitive, versatile, and specific.

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