Luffin-S—a small novel ribosome-inactivating protein from Luffa cylindrica: Characterization and mechanism studies
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Add time:08/15/2019 Source:sciencedirect.com
We purified and characterized a novel RIP (ribosome inactivating protein), Luffin-S from the seeds of Luffa cylindrica. Different from Luffin-A and B, which are RNA N-glycosidases with molecular weights of 27 and 28 kDa, respectively, Luffin-S has an M.W. of only approx. 10 kDa, much smaller than any other RIPs so far investigated. Its abundant resources, toxicity similar to TCS in a cell-free protein synthesis system and unique mechanism as phosphodiesterase, like α-sarcin, promisingly make it a potential toxic moiety of immunotoxin.
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